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Literature summary extracted from

  • Aertgeerts, K.; Levin, I.; Shi, L.; Snell, G.P.; Jennings, A.; Prasad, G.S.; Zhang, Y.; Kraus, M.L.; Salakian, S.; Sridhar, V.; Wijnands, R.; Tennant, M.G.
    Structural and kinetic analysis of the substrate specificity of human fibroblast activation protein alpha (2005), J. Biol. Chem., 280, 19441-19444.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B28 into a modified pFastBacHTb vector Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.B28 at 4°C, by Syrrx´s automated Nanovolume crystallization technology, Ala657 reduces the acidity in this pocket, which may explain the lower affinity for N-terminal amines Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.B28 A657D shows a 60fold increase in the catalytic efficiency for the cleavage of dipeptide substrates, catalytic efficiency reduced by ca. 350fold for the cleavage of benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B28 90000
-
 theoretical value of FAPalpha-His6tag Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B28 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B28 standard protocol Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B28 Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O
-
 Homo sapiens Ala-Pro + 7-amino-4-trifluoromethylcoumarin
-
 ?
3.4.21.B28 benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Gly-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B28 Gly-Pro-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Gly-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B28 Gly-Pro-7-amido-4-trifluoromethylcoumarin + H2O
-
 Homo sapiens Gly-Pro + 7-amino-4-trifluoromethylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.B28 homodimer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B28 FAPalpha
-
 Homo sapiens